Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease
Article Title: LIM Domain Only 2 Regulates Endothelial Proliferation, Angiogenesis, and Tissue Regeneration
doi: 10.1161/JAHA.116.004117
Figure Lengend Snippet: LMO 2 knockdown (KD) impaired endothelial network formation, which can be rescued by exogenous transforming growth factor‐β1 ( TGF‐ β1). A, Network formation of control (CT ) and LMO 2 KD human umbilical vein endothelial cells (HUVECs). B, List of downregulated genes identified in LMO 2 KD in comparison to CT HUVECs using angiogenesis polymerase chain reaction (PCR) array. C, Reverse transcription PCR (RT ‐ PCR ) of TGF‐β 1. D, ELISA for TGF‐ β levels in culture medium of CT and LMO 2 KD cells. E, RT ‐ PCR of TGF‐β 1 in CT and LMO 2 overexpression HUVEC s. F, Network formation of CT and LMO 2 KD HUVECs with or without supplementation of TGF‐ β 10 ng/ mL . G, Chromatin immunoprecipitation PCR analysis of LMO 2 complex binding to TGF‐β 1 promoter (–901 and –4030 region) in both CT and LMO 2 KD cells. Data are presented as mean± SEM (n=3). * P <0.05 vs CT.
Article Snippet: Angiogenesis RT Profiler PCR Array and chromatin immunoprecipitation (ChIP) polymerase chain reaction (PCR) primers were from Qiagen (Valencia, CA).
Techniques: Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Over Expression, Chromatin Immunoprecipitation, Binding Assay